Lesson 26

Children's airborne bacterial infections: diphtheria, scarlet fever, meningococcal infection

Key points:
1. Diphtheria, scarlet fever, meningococcal infection, whooping cough: morphogenesis,morphological characteristics, complications, causes of death.

Sources:
1. Kumar, Vinay, Abul K. Abbas, and Jon C. Aster. Robbins and Cotran Pathologic Basis of Disease. Ninth edition. Philadelphia, PA: Elsevier/Saunders, 2015. P.15-29, 34-38, 66, 100-112
2. Kumar, Vinay, Abul K Abbas, Jon C Aster, and Stanley L. 1915-2003 Robbins. RobbinsBasic Pathology. Tenth edition. Philadelphia, PA: Elsevier/Saunders, 2018. P.19-28, 48-51, 54,87-94
3. Klatt, Edward C. Robbins and Cotran Atlas of Pathology. Third edition. Philadelphia, PA:Elsevier Saunders, 2015.
4. Klatt, Edward C., and Vinay Kumar. Robbins and Cotran Review of Pathology. Fourth edition. Philadelphia, PA: Elsevier Saunders, 2015. P.18-30
5. Harsh Mohan. Essential Pathology for Dental Students.Fourth edition. Jaypee Brothers,Medical Publishers Pvt. Limited, 2011.P. 221-227, 129-137 

Diphtheritic myocarditis, microscopic


Extensive areas of necrosis and degeneration of cardiomyocytes with active inflammation in the interstitial spaces. Numerous large mononuclear cells with eosinophilic cytoplasm are present within these areas

Acute meningitis, microscopic


A neutrophilic exudate involves the meninges on the right, with prominent dilated vessels. Edema and focal inflammation (extending into superficial brain parenchyma through the Virchow-Robin space) are present in the neocortex to the left. This acute meningitis is typical of a bacterial infection. This edema can lead to brain swelling with herniation and death. Resolution of infection may be followed by adhesive arachnoiditis with obliteration of the subarachnoid space leading to obstructive hydrocephalus. Diagnosis is aided by performing lumbar puncture to obtain CSF that typically shows increased leukocytes, mainly neutrophils, decreased glucose, and increased protein. Gram stain, serologies, and culture help identify specific microorganisms.

Streptococcal tonsillitis, microscopic


Extensive purulent neutrophilic exudate is present in the necrotized squamous epithelium of tonsil. Significant inflammatory infiltrate and hyperemia are notable in submucosa. There is hyperplasia of lymphoid follicles with the formation of germinative centres

Diphtheria


Membrane of diphtheria lying within a transverse bronchus. Inhaled C. diphtheriae carried in respiratory droplets proliferate at the site of attachment on the mucosa of the nasopharynx, oropharynx, larynx, or trachea. The bacteria also form satellite lesions in the esophagus or lower airways. Release of exotoxin causes necrosis of the epithelium, accompanied by an outpouring of a dense fibrinosuppurative exudate. The coagulation of this exudate on the ulcerated necrotic surface creates a tough, dirty gray to black, superficial membrane, sometimes called pseudo-membrane because it is not formed by viable tissue

Mechanism of action of DT


DT targets an elongation factor-2 (EF-2). ADP-ribosylation prevents EF-2 tRNA-mRNA translocation activity, resulting in inhibition of protein production and host cell death

Pertussis


Whooping cough showing a haze of bacilli entangled with the cilia of bronchial epithelial cells. The inset highlights the haze of bacilli by immunohistochemistry using a monoclonal antibody reactive to the lipooligosaccharide A of Bordetella pertussis

Acute meningitis, gross 


The yellow-tan clouding of the meninges over gyri shown here, which obscures the sulci, is caused by an inflammatory exudate from acute meningitis. This is most often the result of a bacterial (pyogenic) infection. Routes for intracranial infection include hematogenous dissemination (the most common cause), extension from an adjacent paranasal sinus or mastoid air cells, retrograde flow through facial veins into the cavernous sinus, and trauma with direct implantation by a penetrating injury through the skull. Lumbar puncture reveals increased ICP and CSF showing a marked leukocytosis with a preponderance of neutrophils. Patients often have headache, nuchal rigidity, and changes in mental status.

Waterhouse-Friderichsen syndrome 


These adrenals have a dark-red to black color from extensive hemorrhage with disseminated intravascular coagulopathy as a consequence of endotoxin release from Neisseria meningitidis organisms causing septicemia. This condition is known as Waterhouse-Friderichsen syndrome and is more likely to complicate infections in children. Infection with N. meningitidis can start initially as a mild pharyngitis but become a florid septicemia with hypotension and shock within hours. Destruction of more than 90% of the adrenal cortex leads to adrenal cortical insufficiency.

Scarlet fever


Characteristic strawberry (raspberry) tongue, red cheeks and rash of scarlet fever 

VIRTUAL SLIDES

Case 1: high fever, stiff neck, severe headache with vomiting , photophobia

Case 2: high fever, thick grey-white pharyngeal coating, "bull neck", left ventricle failure

Case 3: high fever, sore throat, red tonsils, and enlarged lymph nodes in the neck